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Beilstein J. Org. Chem. 2023, 19, 1957–1965, doi:10.3762/bjoc.19.146
Graphical Abstract
Figure 1: Workflow for the construction, verification, and expression of the 800 bp GFP gene from chemically ...
Scheme 1: Structure of phosphoramidites 1a,b and 2, and the catching-by-polymerization (CBP) process.
Figure 2: Gel electrophoresis images of 399 bp (A), 401 bp (B), and 800 bp (C) dsODNs derived from synthetic ...
Figure 3: Gel electrophoresis images of ≈399 and ≈401 bp dsODNs originated from synthetic 399 and 401 nt ssOD...
Figure 4: Images of E. coli containing the GFP gene. (A) The GFP gene was derived from the 399 and 401 nt che...
Beilstein J. Org. Chem. 2021, 17, 2976–2982, doi:10.3762/bjoc.17.207
Scheme 1: A comparison of the new PEG synthesis method with a typical known PEG synthesis method.
Scheme 2: Screening base-labile protecting groups for stepwise PEG synthesis. All compounds (3a–l) underwent ...
Scheme 3: Synthesis of monomer 2.
Scheme 4: PEG synthesis using the one-pot PEG elongation approach.
Scheme 5: A proposed route for the synthesis of long and asymmetric PEGs using a base-labile protecting group....